新霉素ELISA快速檢測試劑盒(檢組織/乳制品，靈敏度0.5ppb，一步法)
新霉素ELISA快速檢測試劑盒
一、原理
本試劑盒采用間接競爭ELISA方法，在微孔條上預包被偶聯抗原，樣本中的殘留物新霉素將和微孔條上預包被的偶聯抗原競爭抗新霉素抗體，加入酶標記物后，用TMB底物顯色，樣本吸光值與其所含殘留物新霉素的含量成負相關，與標準曲線比較即可得出相應殘留物新霉素的含量。
二、試劑盒特性
u 試劑盒靈敏度： 0.5ppb
u 孵育溫度： 25℃
u 孵育時間： 60min～15min
u 樣本檢測下限
組織（雞肉/肝、豬肉/肝、蝦、魚 ········· 10ppb
蜂 蜜 ········································· 10ppb
牛奶、奶粉······································· 10ppb
u 交叉反應率
新霉素 ········································· 100%
鏈霉素 ········································· ﹤0.1%
慶大霉素 ······································ ﹤0.1%
雙氫鏈霉素 ··································· ﹤0.1%
卡那霉素 ······································ ﹤0.1%
妥布霉素 ······································ ﹤0.1%
紫蘇霉素 ······································ ﹤0.1%
u 樣本回收率
組織（雞/豬肉、蜂蜜、蝦、魚）·········· 85±19%
雞肝、豬肝 ··································· 70±17%
牛奶、奶粉 ··································· 85±16%
三、試劑盒組成
1 | 微量測試孔 | 每條8孔，一板12條 | |
2 | 標準液×6瓶 （1ml/瓶） | 0ppb | 0.5ppb |
1.5ppb | 4.5ppb | ||
13.5ppb | 40.5ppb | ||
3 | 酶標記物 | 7ml | 紅色帽 |
4 | 抗體工作液 | 7ml | 藍色帽 |
5 | 底物A液 | 7ml | 白色帽 |
6 | 底物B液 | 7ml | 黑色帽 |
7 | 終止液 | 7ml | 黃色帽 |
8 | 20X濃縮洗滌液 | 40ml | 白色帽 |
9 | 2X濃縮復溶液 | 50ml | 透明帽 |
四、樣品前處理及檢測步驟
詳見產品說明書。
五、儲藏條件和保質期
1、 儲藏條件：試劑盒于2～8℃保存，不要冷凍。
2、 保 質 期：該產品有效期為1年，生產日期見包裝盒。
